human breast cancer cell line hs578t Search Results


hs578t  (ATCC)
97
ATCC hs578t
Hs578t, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+breast+cancer+cell+line+hs578t/Hs+578T/10__1158_slash_2767___9764__crc___23___0236-48-7-8
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hs578t cells  (LGC Standards)
93
LGC Standards hs578t cells
Cells were treated with 500 ng/ml CCL21 for 0, 1, 2, 5, and 10 minutes. For inhibitor studies cells were preincubated for 60 minutes with 500 nM Ly29004 or 30 minutes with 500 nM PD98059, respectively. Cells were analyzed for AKT phosphorylation and MAPK p42/44 (MAPK) phosphorylation, whereby elf4E was used as a housekeeping gene. Values indicate the relative intensities of proteins in relation to elf4E, which was set to 100%. Shown are representative Western Blot data of n = 3 independent experiments. (A) <t>HS578T-Hyg</t> breast cancer cells, (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells.
Hs578t Cells, supplied by LGC Standards, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+breast+cancer+cell+line+hs578t/Animal+Bone/pmc03646822-45-10-13
Average 93 stars, based on 1 article reviews
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hs 578t human tnbc cell lines  (Korean Cell Line Bank)
86
Korean Cell Line Bank hs 578t human tnbc cell lines
Cells were treated with 500 ng/ml CCL21 for 0, 1, 2, 5, and 10 minutes. For inhibitor studies cells were preincubated for 60 minutes with 500 nM Ly29004 or 30 minutes with 500 nM PD98059, respectively. Cells were analyzed for AKT phosphorylation and MAPK p42/44 (MAPK) phosphorylation, whereby elf4E was used as a housekeeping gene. Values indicate the relative intensities of proteins in relation to elf4E, which was set to 100%. Shown are representative Western Blot data of n = 3 independent experiments. (A) <t>HS578T-Hyg</t> breast cancer cells, (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells.
Hs 578t Human Tnbc Cell Lines, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+breast+cancer+cell+line+hs578t/578t+hs/pm41463012-66-2-12
Average 86 stars, based on 1 article reviews
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human breast cancer cell lines  (ATCC)
99
ATCC human breast cancer cell lines
Cells were treated with 500 ng/ml CCL21 for 0, 1, 2, 5, and 10 minutes. For inhibitor studies cells were preincubated for 60 minutes with 500 nM Ly29004 or 30 minutes with 500 nM PD98059, respectively. Cells were analyzed for AKT phosphorylation and MAPK p42/44 (MAPK) phosphorylation, whereby elf4E was used as a housekeeping gene. Values indicate the relative intensities of proteins in relation to elf4E, which was set to 100%. Shown are representative Western Blot data of n = 3 independent experiments. (A) <t>HS578T-Hyg</t> breast cancer cells, (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells.
Human Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+breast+cancer+cell+line+hs578t/MCF7/pmc09916895-123-0-16
Average 99 stars, based on 1 article reviews
human breast cancer cell lines - by Bioz Stars, 2026-09
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human bc cell lines  (ATCC)
99
ATCC human bc cell lines
Cells were treated with 500 ng/ml CCL21 for 0, 1, 2, 5, and 10 minutes. For inhibitor studies cells were preincubated for 60 minutes with 500 nM Ly29004 or 30 minutes with 500 nM PD98059, respectively. Cells were analyzed for AKT phosphorylation and MAPK p42/44 (MAPK) phosphorylation, whereby elf4E was used as a housekeeping gene. Values indicate the relative intensities of proteins in relation to elf4E, which was set to 100%. Shown are representative Western Blot data of n = 3 independent experiments. (A) <t>HS578T-Hyg</t> breast cancer cells, (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells.
Human Bc Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+breast+cancer+cell+line+hs578t/T-47D/pmc11700287-53-8-22
Average 99 stars, based on 1 article reviews
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breast  (ATCC)
99
ATCC breast
Cells were treated with 500 ng/ml CCL21 for 0, 1, 2, 5, and 10 minutes. For inhibitor studies cells were preincubated for 60 minutes with 500 nM Ly29004 or 30 minutes with 500 nM PD98059, respectively. Cells were analyzed for AKT phosphorylation and MAPK p42/44 (MAPK) phosphorylation, whereby elf4E was used as a housekeeping gene. Values indicate the relative intensities of proteins in relation to elf4E, which was set to 100%. Shown are representative Western Blot data of n = 3 independent experiments. (A) <t>HS578T-Hyg</t> breast cancer cells, (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells.
Breast, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+breast+cancer+cell+line+hs578t/MDA-MB-231/pmc11427869-528-25-26
Average 99 stars, based on 1 article reviews
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tnbc  (ATCC)
97
ATCC tnbc
Cells were treated with 500 ng/ml CCL21 for 0, 1, 2, 5, and 10 minutes. For inhibitor studies cells were preincubated for 60 minutes with 500 nM Ly29004 or 30 minutes with 500 nM PD98059, respectively. Cells were analyzed for AKT phosphorylation and MAPK p42/44 (MAPK) phosphorylation, whereby elf4E was used as a housekeeping gene. Values indicate the relative intensities of proteins in relation to elf4E, which was set to 100%. Shown are representative Western Blot data of n = 3 independent experiments. (A) <t>HS578T-Hyg</t> breast cancer cells, (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells.
Tnbc, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+breast+cancer+cell+line+hs578t/HCC1806/pm33288557-40-9-30
Average 97 stars, based on 1 article reviews
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hs 578t  (ATCC)
95
ATCC hs 578t
Cells were treated with 500 ng/ml CCL21 for 0, 1, 2, 5, and 10 minutes. For inhibitor studies cells were preincubated for 60 minutes with 500 nM Ly29004 or 30 minutes with 500 nM PD98059, respectively. Cells were analyzed for AKT phosphorylation and MAPK p42/44 (MAPK) phosphorylation, whereby elf4E was used as a housekeeping gene. Values indicate the relative intensities of proteins in relation to elf4E, which was set to 100%. Shown are representative Western Blot data of n = 3 independent experiments. (A) <t>HS578T-Hyg</t> breast cancer cells, (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells.
Hs 578t, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+breast+cancer+cell+line+hs578t/Hs+578T%3B+Breast+Carcinoma%3B+Human/us07385061-310-89-91
Average 95 stars, based on 1 article reviews
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hs 578t  (DSMZ)
94
DSMZ hs 578t
Cells were treated with 500 ng/ml CCL21 for 0, 1, 2, 5, and 10 minutes. For inhibitor studies cells were preincubated for 60 minutes with 500 nM Ly29004 or 30 minutes with 500 nM PD98059, respectively. Cells were analyzed for AKT phosphorylation and MAPK p42/44 (MAPK) phosphorylation, whereby elf4E was used as a housekeeping gene. Values indicate the relative intensities of proteins in relation to elf4E, which was set to 100%. Shown are representative Western Blot data of n = 3 independent experiments. (A) <t>HS578T-Hyg</t> breast cancer cells, (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells.
Hs 578t, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+breast+cancer+cell+line+hs578t/HS-578T/pm41814308-61-28-26
Average 94 stars, based on 1 article reviews
hs 578t - by Bioz Stars, 2026-09
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human bc cells  (ATCC)
97
ATCC human bc cells
Cells were treated with 500 ng/ml CCL21 for 0, 1, 2, 5, and 10 minutes. For inhibitor studies cells were preincubated for 60 minutes with 500 nM Ly29004 or 30 minutes with 500 nM PD98059, respectively. Cells were analyzed for AKT phosphorylation and MAPK p42/44 (MAPK) phosphorylation, whereby elf4E was used as a housekeeping gene. Values indicate the relative intensities of proteins in relation to elf4E, which was set to 100%. Shown are representative Western Blot data of n = 3 independent experiments. (A) <t>HS578T-Hyg</t> breast cancer cells, (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells.
Human Bc Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+breast+cancer+cell+line+hs578t/SK-BR-3/pmc10408694-44-0-20
Average 97 stars, based on 1 article reviews
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breast cancer cell lines  (ATCC)
97
ATCC breast cancer cell lines
Cells were treated with 500 ng/ml CCL21 for 0, 1, 2, 5, and 10 minutes. For inhibitor studies cells were preincubated for 60 minutes with 500 nM Ly29004 or 30 minutes with 500 nM PD98059, respectively. Cells were analyzed for AKT phosphorylation and MAPK p42/44 (MAPK) phosphorylation, whereby elf4E was used as a housekeeping gene. Values indicate the relative intensities of proteins in relation to elf4E, which was set to 100%. Shown are representative Western Blot data of n = 3 independent experiments. (A) <t>HS578T-Hyg</t> breast cancer cells, (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells.
Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+breast+cancer+cell+line+hs578t/HCC1937/10__1097_slash_cad__0000000000000897-41-4-28
Average 97 stars, based on 1 article reviews
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Image Search Results


Cells were treated with 500 ng/ml CCL21 for 0, 1, 2, 5, and 10 minutes. For inhibitor studies cells were preincubated for 60 minutes with 500 nM Ly29004 or 30 minutes with 500 nM PD98059, respectively. Cells were analyzed for AKT phosphorylation and MAPK p42/44 (MAPK) phosphorylation, whereby elf4E was used as a housekeeping gene. Values indicate the relative intensities of proteins in relation to elf4E, which was set to 100%. Shown are representative Western Blot data of n = 3 independent experiments. (A) HS578T-Hyg breast cancer cells, (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells.

Journal: PLoS ONE

Article Title: Fusion of CCL21 Non-Migratory Active Breast Epithelial and Breast Cancer Cells Give Rise to CCL21 Migratory Active Tumor Hybrid Cell Lines

doi: 10.1371/journal.pone.0063711

Figure Lengend Snippet: Cells were treated with 500 ng/ml CCL21 for 0, 1, 2, 5, and 10 minutes. For inhibitor studies cells were preincubated for 60 minutes with 500 nM Ly29004 or 30 minutes with 500 nM PD98059, respectively. Cells were analyzed for AKT phosphorylation and MAPK p42/44 (MAPK) phosphorylation, whereby elf4E was used as a housekeeping gene. Values indicate the relative intensities of proteins in relation to elf4E, which was set to 100%. Shown are representative Western Blot data of n = 3 independent experiments. (A) HS578T-Hyg breast cancer cells, (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells.

Article Snippet: HS578T-Hyg breast cancer cells were generated by stable transfection of HS578T cells (HTB-126; LGC Standards GmbH, Wesel, Germany) with pKS-Hyg.

Techniques: Phospho-proteomics, Western Blot

The migratory activity was analyzed using the 3D collagen matrix migration assay combined with time-lapse video-microscopy. For a better comparison the locomotory activities of CCL21 stimulated cells were calculated in relation to untreated control cells, which were set to 100%. Cells were stimulated with 500 ng/ml CCL21. Shown are the mean±SD of n = 3 independent experiments. (A) HS578T-Hyg breast cancer cells, (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells. Statistical significance was calculated using Student's t -test: *** = p <0.001.

Journal: PLoS ONE

Article Title: Fusion of CCL21 Non-Migratory Active Breast Epithelial and Breast Cancer Cells Give Rise to CCL21 Migratory Active Tumor Hybrid Cell Lines

doi: 10.1371/journal.pone.0063711

Figure Lengend Snippet: The migratory activity was analyzed using the 3D collagen matrix migration assay combined with time-lapse video-microscopy. For a better comparison the locomotory activities of CCL21 stimulated cells were calculated in relation to untreated control cells, which were set to 100%. Cells were stimulated with 500 ng/ml CCL21. Shown are the mean±SD of n = 3 independent experiments. (A) HS578T-Hyg breast cancer cells, (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells. Statistical significance was calculated using Student's t -test: *** = p <0.001.

Article Snippet: HS578T-Hyg breast cancer cells were generated by stable transfection of HS578T cells (HTB-126; LGC Standards GmbH, Wesel, Germany) with pKS-Hyg.

Techniques: Activity Assay, Migration, Microscopy, Comparison, Control

The migratory activity was analyzed using the 3D collagen matrix migration assay combined with time-lapse video-microscopy. For a better comparison the locomotory activities of CCL21 and inhibitor stimulated cells were calculated in relation to untreated control cells, which were set to 100%. Cells were treated with 500 ng/ml CCL21, 5 µM U73122, 500 nM Ly294002, and 500 nM PD98059. Shown are the mean±SD of n = 4 independent experiments. (A) HS578T-Hyg breast cancer cells (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells. Statistical significance was calculated using Student's t -test: “*” = statistical significance vs. control: ** = p <0.01; *** = p <0.001. “+” = statistical significance vs. CCL21: ++ = p <0.01; +++ = p <0.001.

Journal: PLoS ONE

Article Title: Fusion of CCL21 Non-Migratory Active Breast Epithelial and Breast Cancer Cells Give Rise to CCL21 Migratory Active Tumor Hybrid Cell Lines

doi: 10.1371/journal.pone.0063711

Figure Lengend Snippet: The migratory activity was analyzed using the 3D collagen matrix migration assay combined with time-lapse video-microscopy. For a better comparison the locomotory activities of CCL21 and inhibitor stimulated cells were calculated in relation to untreated control cells, which were set to 100%. Cells were treated with 500 ng/ml CCL21, 5 µM U73122, 500 nM Ly294002, and 500 nM PD98059. Shown are the mean±SD of n = 4 independent experiments. (A) HS578T-Hyg breast cancer cells (B) M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics, (C) M13HS-2 hybrid cells, (D) M13HS-8 hybrid cells. Statistical significance was calculated using Student's t -test: “*” = statistical significance vs. control: ** = p <0.01; *** = p <0.001. “+” = statistical significance vs. CCL21: ++ = p <0.01; +++ = p <0.001.

Article Snippet: HS578T-Hyg breast cancer cells were generated by stable transfection of HS578T cells (HTB-126; LGC Standards GmbH, Wesel, Germany) with pKS-Hyg.

Techniques: Activity Assay, Migration, Microscopy, Comparison, Control